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KMID : 0545120010110040685
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Journal of Microbiology and Biotechnology
2001 Volume.11 No. 4 p.685 ~ p.692
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Cloning of a Paenibacillus sp. Endo-β-1,4-Glucanase Gene and Its Coexpression with the Endomyces fibuliger β-Glucosidase Gene in Saccharomyces cerevisiae
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PARK, JEONG-NAM
KIM, HEE-OK/SHIN, DONG-JUN/KIM, HYO-JEONG/LEE, HWANGHEE BLAISE/CHUN, SOON-BAI/BAI, SUK
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Abstract
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A gene, Egl, from Paenibacillus sp. KCTC 8848P encoding endo-β-1,4-glucanase was cloned and expressed in Escherichia coli. It consisted of an open reading frame of 1,191bp for a protein that consisted of 397 amino acids with a molecular weight of 44,539Da. The deduced amino acid sequence of the endo-β-1,4-glucanase gene had a 94% similarity to the endo-β-1,4-glucanase of Bacillus polymyxa. The Egl gene was also expressed in Saccharomyces cerevisiae secreting Endomyces fibuliger β-glucosidase (BGL1) under the control of the alcohol dehydrogenase (ADC1) gene promoter. S. cerevisiae transformant producing both endo-β-1,4-glucanase and β-glucosidase grew on carboxymethyl cellulose as the sole carbon source.
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